Continuous monitoring of sterility: Bacterial contamination and disinfectant performance in dental clinics
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Vol 16 No 1 (2025), Original Article
Vol 16 No 1 (2025)

Continuous monitoring of sterility: Bacterial contamination and disinfectant performance in dental clinics

Original Article
https://doi.org/10.70174/iajd.v16i1.1395
Published 7 April 2025
Tarek ABOU AGWA
Oral and maxillofacial surgery Department, Al-Wataniya Private University, Hama, Syria.
Shuaib ALAHMAD
Pharmaceutical Analytical Chemistry Department, Al-Wataniya Private University, Hama, Syria.
Nermin ALMOUHAMMAD
Biochemistry and Microbiology Department, Al-Wataniya Private University, Hama, Syria. Supervisor of premarital laboratory in Homs-Syria.
Nathaly ALSALLOUM
Biochemistry and Microbiology Department, Al-Wataniya Private University, Hama, Syria. Hama Health Directorate.
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Keywords

Dental clinic
Bacterial contamination
Disinfectant activity
Bacterial species

How to Cite

ABOU AGWA, T., ALAHMAD, S., ALMOUHAMMAD, N., & ALSALLOUM, N. (2025). Continuous monitoring of sterility: Bacterial contamination and disinfectant performance in dental clinics. International Arab Journal of Dentistry (IAJD), 16(1), 95-106. https://doi.org/10.70174/iajd.v16i1.1395
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Abstract

Objectives: This study aimed to (1) evaluate contamination levels across three clinics (endodontic, extraction and surgery, and fixed prosthodontics), (2) identify the types and prevalence of bacteria, (3) estimate the effectiveness of a commercial disinfectant, and (4) determine the bacterial species most responsive to the disinfectant.

Methods: Equal surfaces (17.98 cm²) were swabbed, cultured on nutrient agar for the total aerobic microbial count before and after disinfection, and analyzed using settle plates. Bacterial identification was conducted through colony characteristics, Gram staining, and biochemical tests. Data were processed using SPSS version 26.

Results: The endodontic clinic exhibited the highest contamination levels, with buttons significantly more contaminated than handles. The disinfectant effectively reduced contamination but did not eliminate it.

Conclusions: Contamination levels varied significantly between the clinics; ongoing evaluation is essential.

https://doi.org/10.70174/iajd.v16i1.1395
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